Tool
Melting temperature and GC content
Nearest-neighbour Tm at the salt and strand concentration you actually work at, with GC%, length, and the published corrections for modified residues.
Sequence (5' → 3')
Loading sequence editor…
Modified residues count. LNA is scored with its published nearest-neighbour parameters, and 2′-OMe, MOE, 2′-F and phosphorothioate with their published ΔTm.